DNA PROTOCOLS |
| Agarose gel electrophoresis (185) |
Genomic DNA isolation from other sources (under development) |
| Alkaline Phosphatase removal of PO4 from DNA fragments (153) |
Modification of DNA ends (110) |
| cDNA amplification from l-phage library (189) |
PCR amplification and applications (146) |
| cDNA library screening (144) |
PCR product purification (194) |
| DIG DNA labeling and hybrid detection (193) |
Perfectly blunt cloning of DNA (190) |
| DNA Amplification of specific target cDNA by PCR (142) |
Plasmid midi-prep from bacteria (101) |
| DNA extraction from agarose (195) |
Plasmid mini-prep from bacteria (109) |
| DNA fragment isolation from low melting agarose (108) |
Random-primed labeling of DNA (114) |
| DNA ligation (131) |
Sequencing of DNA using Sequenase (149) |
| Genomic DNA isolation from plants (115) |
Southern blot (166) |
| Genomic DNA isolation from animals (under development) |
Wizard plus mini-prep from bacteria (107) |
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RNA PROTOCOLS |
| Affymetrix Microarray Target Hybridization (147) |
RNA and protein extraction from the same tissue (139) |
| In vitro transcription (140) |
RNA extraction from plant tissues (119) |
| Isolation of poly-A RNA (141) |
RNA extraction from animal tissues (under development) |
| Northern blot (113) |
Washing, Staining, and Scanning Affymetrix Microarrays (152) |
| Probe labeling for Affymetrix microarrays (143) |
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PROTEIN PROTOCOLS |
| Affinity column preparation and immunoprecipitation (126) |
Immunoprecipitation (156) |
| Affinity purification of antibodies (121) |
Membrane separation (122) |
| Bradford quantification of protein concentration (192) |
Polyclonal antibody preparation and storage (132) (under development) |
| B-PER bacterial protein extraction (186) |
Protein precipitations (TCA, Methanol, Ammonium Sulphate) (145)
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| Cross-linking of proteins (178) (under development) |
Protein preparation from plant tissues (120) |
| Electroelution (129) (under development) |
Protein staining (137) |
| Factor Xa cleavage of GST protein fusions (150) |
Radiolabeling of protoplasts (105) |
| GST-fusion protein injection into rabbits (154) |
SDS/Native PAGE (112) |
| GST-fusion protein expression and purification (111) |
Western blot (118) |
| His-fusion protein purification (188) |
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BIOCHEMISTRY PROTOCOLS |
| ATPase reaction (activated by potassium) (175) (under development) |
Size exclusion column (P2) (179) |
| ATPase reaction (vanadate sensitive) (176) (under development) |
Transport of UDP-Glc to Golgi vesicles (172) |
| Golgi vesicle preparation from pea etiolated hypocotyls (171) |
Tritiated-UDP-Glc synthesis (173) (under development) |
| IDPase reaction (177) (under development) |
UDPase reaction (174) (under development) |
| Lipid extraction (206) |
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CARBOHYDRATE PROTOCOLS |
| Cell wall purification from Arabidopsis tissues (103) |
Thin layer chromatography (158) |
| Fucosyltransferase assay (104) |
UDP-sugar binding assay for RGP (116) |
| GC analysis of alditol acetates (165) |
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BACTERIA PROTOCOLS |
| Electroporation (162) |
Introduction of plasmid into Agrobacterium III (196) |
| Electro-competent Agrobacterium cells (148) |
Preparation of competent E. coli cells using calcium chloride (135) |
| Introduction of plasmid into Agrobacterium II (160) |
Transformation of bacteria (133) |
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PLANT PROTOCOLS |
| Arabidopsis cell suspension and root liquid culture (102) |
Preparation of pea seeds for germination and growth (181) |
| Arabidopsis flower preparation for fertilization (169) |
Protoplast preparation from plant tissues (128) |
| Arabidopsis root culture (138) |
Vacuum infiltration of Arabidopsis plants (161) |
| Cross-fertilization of Arabidopsis (170) |
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ANIMAL PROTOCOLS |
| Cell culture of mouse myoblasts (106) |
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BUFFERS AND STANDARD SOLUTIONS |
| Buffers and Standard Solutions (151) |
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OTHER PROTOCOL PAGES |
| Properties of detergents (199) |
Methods and Reagents |
| TAIR |
Protein Crystallography (Birkbeck College) |
| Molecular Biology Today |
Cell Biology Laboratory Manual |
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SCIENCE TOOLS PAGES |
| Fun Biology Activities for Kids |
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