DNA PROTOCOLS
Agarose gel electrophoresis (185)
Genomic DNA isolation from other sources (under development)
Alkaline Phosphatase removal of PO4 from DNA fragments (153)
Modification of DNA ends (110)
cDNA amplification from
l
-phage library (189)
PCR amplification and applications (146)
cDNA library screening (144)
PCR product purification (194)
DIG DNA labeling and hybrid detection (193)
Perfectly blunt cloning of DNA (190)
DNA Amplification of specific target cDNA by PCR (142)
Plasmid midi-prep from bacteria (101)
DNA extraction from agarose (195)
Plasmid mini-prep from bacteria (109)
DNA fragment isolation from low melting agarose (108)
Random-primed labeling of DNA (114)
DNA ligation (131)
Sequencing of DNA using Sequenase (149)
Genomic DNA isolation from plants (115)
Southern blot (166)
Genomic DNA isolation from animals (under development)
Wizard plus mini-prep from bacteria (107)
RNA PROTOCOLS
Affymetrix Microarray Target Hybridization (147)
RNA and protein extraction from the same tissue (139)
In vitro transcription (140)
RNA extraction from plant tissues (119)
Isolation of poly-A RNA (141)
RNA extraction from animal tissues (under development)
Northern blot (113)
Washing, Staining, and Scanning Affymetrix Microarrays (152)
Probe labeling for Affymetrix microarrays (143)
PROTEIN PROTOCOLS
Affinity column preparation and immunoprecipitation (126)
Immunoprecipitation (156)
Affinity purification of antibodies (121)
Membrane separation (122)
Bradford quantification of protein concentration (192)
Polyclonal antibody preparation and storage (132) (under development)
B-PER bacterial protein extraction (186)
Protein precipitations (TCA, Methanol, Ammonium Sulphate) (145)
Cross-linking of proteins (178) (under development)
Protein preparation from plant tissues (120)
Electroelution (129) (under development)
Protein staining (137)
Factor Xa cleavage of GST protein fusions (150)
Radiolabeling of protoplasts (105)
GST-fusion protein injection into rabbits (154)
SDS/Native PAGE (112)
GST-fusion protein expression and purification (111)
Western blot (118)
His-fusion protein purification (188)
BIOCHEMISTRY PROTOCOLS
ATPase reaction (activated by potassium) (175) (under development)
Size exclusion column (P2) (179)
ATPase reaction (vanadate sensitive) (176) (under development)
Transport of UDP-Glc to Golgi vesicles (172)
Golgi vesicle preparation from pea etiolated hypocotyls (171)
Tritiated-UDP-Glc synthesis (173) (under development)
IDPase reaction (177) (under development)
UDPase reaction (174) (under development)
Lipid extraction (206)
CARBOHYDRATE PROTOCOLS
Cell wall purification from Arabidopsis tissues (103)
Thin layer chromatography (158)
Fucosyltransferase assay (104)
UDP-sugar binding assay for RGP (116)
GC analysis of alditol acetates (165)
BACTERIA PROTOCOLS
Electroporation (162)
Introduction of plasmid into Agrobacterium III (196)
Electro-competent Agrobacterium cells (148)
Preparation of competent E. coli cells using calcium chloride (135)
Introduction of plasmid into Agrobacterium II (160)
Transformation of bacteria (133)
PLANT PROTOCOLS
Arabidopsis cell suspension and root liquid culture (102)
Preparation of pea seeds for germination and growth (181)
Arabidopsis flower preparation for fertilization (169)
Protoplast preparation from plant tissues (128)
Arabidopsis root culture (138)
Vacuum infiltration of Arabidopsis plants (161)
Cross-fertilization of Arabidopsis (170)
ANIMAL PROTOCOLS
Cell culture of mouse myoblasts (106)
BUFFERS AND STANDARD SOLUTIONS
Buffers and Standard Solutions (151)
OTHER PROTOCOL PAGES
Properties of detergents (199)
Methods and Reagents
TAIR
Protein Crystallography
(Birkbeck College)
Molecular Biology Today
Cell Biology Laboratory Manual
SCIENCE TOOLS PAGES
Fun Biology Activities for Kids
